FastQCFastQC Report
Sat 17 Dec 2016
fastqc_v0101_02.fq

Summary

[OK] Basic Statistics

Measure Value
Filename fastqc_v0101_02.fq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 1000
Filtered Sequences 0
Sequence length 100
%GC 53

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCAAGATCTCGTAT 14 1.4000000000000001 TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCAAGATCTCGTATG 12 1.2 TruSeq Adapter, Index 1 (97% over 36bp)
GGCTTGTTTTATTTTAATGGCTGATCTATGTAATCACAGAGGCCAGTATG 2 0.2 No Hit
AGGGGGAATGATGGTTGTCTTTGGATATACTACAGCGATGGCTATTGAGG 2 0.2 No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTTCCAAGATCTCGTATGCCG 2 0.2 TruSeq Adapter, Index 1 (96% over 33bp)
GTGGGGTGGTGTTTGTGGGGGACTTCATCATCTCAGGCTTCCCAGGGTCC 2 0.2 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 385 7.3597403 68.038994 65-69
AGATC 435 5.4375157 23.135067 1
GAAGA 375 5.258809 32.443344 6
GGAAG 420 5.044668 33.345257 5
TCCAG 475 4.8355613 14.131038 2
AAGAG 320 4.487517 25.954676 7
CCAGG 475 4.4180827 17.21471 3
GAGCA 380 4.3399205 21.1377 9
AGCAC 395 4.2895336 15.0741825 7
CTCCA 415 4.0171337 12.105032 95-96
AGAGC 340 3.883087 21.137697 8
TTTTT 280 3.8749053 8.964593 10-14
CTTCT 370 3.8646336 11.598914 55-59
CTGAA 305 3.812511 13.130004 90-94
CGGAA 320 3.65467 26.422123 5
ACCAG 335 3.6379597 10.049457 7
TCTGA 310 3.6325634 12.308498 90-94
CACAC 340 3.5108058 14.806036 85-89
ATCGG 325 3.4795394 24.768969 3
TCGGA 320 3.426008 19.815174 3
GATCG 320 3.426008 19.815174 1
CGTCT 355 3.387832 11.578538 85-89
CTGCT 355 3.387832 17.662533 3
GCACA 310 3.3664696 15.0741825 8
TCTTC 320 3.3423858 7.7326093 50-54
CAGCA 305 3.3121717 10.049455 6
GAACT 260 3.2500093 13.130004 90-94
GTCTG 320 3.2116532 12.65067 90-94
CAGGA 280 3.197836 15.8532715 3
AACTC 265 3.1497202 23.781752 95-96
TGAAC 250 3.125009 13.130004 90-94
CCAGC 350 3.0954454 6.6359653 95-96
AGTCA 240 3.0000086 10.41078 25-29
CACCA 290 2.9945107 6.079907 70-74
TGCTG 295 2.960743 9.2877 2
CAGAT 230 2.875008 11.040063 70-74
CTTCC 315 2.8583732 10.916445 30-34
CACGT 280 2.8504362 12.351324 85-89
CAGGG 290 2.8367646 22.630535 9
ACACG 260 2.8234906 13.175687 85-89
TTCCA 250 2.7855206 9.279795 30-34
TTCTT 230 2.765239 6.6755276 50-54
AGCAG 240 2.7410026 15.853272 2
TTCTG 240 2.6363494 10.165324 55-59
ACTCC 270 2.6135564 14.526036 95-96
GCCAG 280 2.6043434 8.607355 1
ACGTC 255 2.595933 10.105629 85-89
GATCT 220 2.5779483 8.675031 40-44
TCTGC 265 2.5289452 13.2469015 2
AAGAT 160 2.4557784 12.783248 35-39
ATCTC 220 2.4512577 9.279794 40-44
CAGTC 240 2.4432309 8.554544 90-94
TCCAA 205 2.4365761 10.999062 7
CTTTT 200 2.4045558 16.688818 6
TTCCT 230 2.40234 9.665762 7
CCAGT 235 2.3923304 9.4206915 25-29
TTTCT 195 2.3444414 16.688818 8
CTGGG 255 2.3383298 6.004135 80-84
TGCTT 210 2.3068056 10.165323 4
TCTTT 190 2.284328 5.5629396 15-19
TTTTC 190 2.2843277 11.125878 7
GGGGG 255 2.2468696 16.307867 2
AGGAA 160 2.2437584 19.466007 5
GTCAC 220 2.2396283 10.184532 95-96
TCACT 200 2.2284167 8.360176 95-96
CACTT 200 2.2284167 10.3108835 30-34
GAAAA 135 2.2103586 10.606119 60-64
ACTTC 195 2.172706 9.279794 30-34
TTGAA 150 2.1582448 11.9834385 60-64
CTCCT 235 2.1324375 16.794533 4
TCCTC 235 2.1324372 8.397265 5
ATCTT 165 2.11616 7.1210704 10-14
GGGGA 205 2.1089406 14.2801 3
ACACA 165 2.092039 11.7331705 8
TGCAG 195 2.0877237 9.907587 5
GACCA 190 2.0633202 10.049455 6
AGGGG 200 2.057503 9.520067 1
CCTCC 260 2.049668 14.590484 5
AGGAG 170 2.0418897 5.557543 2
TCCTT 195 2.0367663 14.498643 4
GTCTT 185 2.032186 15.247986 7
GCTGG 220 2.0173824 8.485845 1
CCAGA 185 2.0090222 5.3284492 70-74
CCTGG 230 2.0054333 8.068818 3
GCAGG 205 2.005299 9.052214 3
GGACC 215 1.9997637 8.607355 5
TTCAT 155 1.987908 5.934226 2
CCTTT 190 1.9845415 14.498643 5
TTTCC 190 1.9845415 5.799457 15-19
TGGCA 185 1.980661 14.861383 2
TCTTG 180 1.977262 10.165323 5
CCAAG 180 1.9547247 9.044511 35-39
CTTCA 175 1.9498644 10.310883 6
CAAGA 145 1.933477 12.339583 35-39
CTGGA 180 1.9271295 9.907587 6
GGCTG 210 1.9256833 16.97169 2
AATGA 125 1.918577 7.677627 95-96
TGAAA 125 1.918577 15.623971 60-64
GCTTC 200 1.9086379 13.2469015 2
GTCCA 185 1.8833237 14.131036 1
AGAAA 115 1.882898 7.5757995 7
TGGGG 195 1.8805519 13.386638 1
TTCTC 180 1.880092 5.799457 25-29
CTTGA 160 1.8748715 8.675031 60-64
ACAAA 120 1.8682072 5.762797 40-44
TCTCG 195 1.8609219 8.831266 5
GGGAC 190 1.8585701 9.052216 5
TGAGG 165 1.8578365 5.209824 2
TGAAG 140 1.8404517 6.082693 2
CATCT 165 1.8384434 5.155441 4
CACTG 180 1.8324232 9.4206915 6
CTGCA 180 1.8324231 5.3465896 90-94
GCTGC 210 1.8310483 8.068819 1
GCAGA 160 1.8273348 10.568848 3
CCTTC 200 1.8148402 8.397265 9
AGGGA 150 1.8016673 6.0081544 95-96
TTTCA 140 1.7955297 7.1210704 15-19
CACAG 165 1.7918309 5.432139 95-96
AAACA 115 1.7903653 7.6389136 70-74
ATTTT 120 1.7715117 13.661307 6
TTTTG 140 1.7701824 17.551357 7
GGGGC 210 1.7594293 11.629828 3
GATTT 130 1.7534488 12.481857 6
CAAAT 120 1.7513192 6.7527947 50-54
GAGGG 170 1.7488776 9.520067 1
GAAGG 145 1.7416117 6.0081544 95-96
CATTT 135 1.7314036 5.9342256 5
ATTTC 135 1.7314036 5.9342256 7
CCTCT 190 1.7240983 8.397266 1
ATCCA 145 1.7234317 5.49953 4
GCAGC 185 1.7207267 6.9789357 95-96
TCCTG 180 1.717774 13.2469 2
CTCTG 180 1.717774 13.2469 2
AAAAC 110 1.7125233 7.6389136 70-74
CTTGG 170 1.7061908 9.2877 2
AAAAT 95 1.7024158 8.291661 9
TCACC 175 1.693972 8.957724 8
TCCAC 175 1.693972 8.957724 5
GAGAA 120 1.6828189 6.488669 6
TCTCC 185 1.6787271 5.038359 55-59
GAGCC 180 1.6742208 8.607355 9
TCATC 150 1.6713123 5.1554413 2
AGACA 125 1.6667906 6.169792 2
TGATG 135 1.6636823 11.404236 9
GGGAG 160 1.6460025 9.520067 1
AGCCA 150 1.6289369 6.029673 10-14
ATGCC 160 1.6288207 8.478622 45-49
CTCGT 170 1.6223421 8.831266 3
GAGGA 135 1.6215005 11.115086 3
TGTTG 140 1.6173534 10.690706 2
CTCAT 145 1.6156021 5.1554418 2
CAGGT 150 1.6059413 9.907587 4
GCTTG 160 1.6058266 9.2877 60-64
GGGTC 175 1.6047363 12.728768 2
TCATT 125 1.6031516 5.934226 9
GTTGA 130 1.6020645 5.702118 1
ACAGA 120 1.6001189 10.005068 95-96
GGAGG 155 1.5945649 9.520067 2
GGGGT 165 1.5912362 13.386638 1
TGGGA 140 1.5763463 10.419649 2
GGATG 140 1.5763462 15.629472 6
GCCTC 190 1.575248 7.672287 2
CCTGC 190 1.5752479 11.508429 2
GCTCC 190 1.5752479 11.508429 6
TCTCT 150 1.5667434 5.224736 95-96
GGGAA 130 1.561445 11.115086 4
TCCAT 140 1.5598917 10.3108835 8
GGCTT 155 1.5556445 13.93155 1
TTGAT 115 1.5511277 6.240928 4
CATCA 130 1.5451456 5.49953 2
AGAGA 110 1.542584 6.488669 9
AGGAC 135 1.541814 6.341309 55-59
GTATG 125 1.5404466 9.123388 45-49
AACAT 105 1.5324043 13.5055895 9
AGCTC 150 1.5270194 9.4206915 5
TTTGT 120 1.5172992 17.551357 8
GATGA 115 1.5117996 6.082693 5
GAGAT 115 1.5117996 6.082693 4
AGGAT 115 1.5117996 12.165386 4
TGAGA 115 1.5117996 6.082693 5
CTGGT 150 1.5054625 9.2877 4
GCTGT 150 1.5054625 18.5754 3
TTCAC 135 1.504181 10.310883 7
CCCAG 170 1.5035021 12.276537 2
CAGTG 140 1.4988785 9.907587 5
CTCCC 190 1.4978343 7.295242 1
CCCTG 180 1.4923402 11.5084305 2
CAGAG 130 1.4847097 7.398194 20-24
CTTTG 135 1.4829465 10.165323 2
CAAAA 95 1.4789973 7.203496 9
TCTCA 130 1.4484707 5.1554413 8
GAATG 110 1.4460692 12.165386 7
GGAAT 110 1.4460692 12.165386 5
TTTGG 125 1.4440656 5.345353 7
GGCCT 165 1.4386805 12.103227 1
GCTCT 150 1.4314783 6.1818867 20-24
TCTGT 130 1.4280226 15.247986 3
CTGTT 130 1.4280226 15.247986 4
AGGTT 115 1.4172109 11.404235 8
TTGAG 115 1.4172107 5.702117 4
TTTGA 105 1.416247 7.4891143 10-14
ATCTG 120 1.4061534 5.4218936 2
GGTCT 140 1.4050984 9.287701 6
TTTTA 95 1.4024467 7.384491 95-96
GGGTG 145 1.3983592 13.386638 2
GGCAC 150 1.3951839 8.607355 4
AAAGA 85 1.3917071 7.5757985 8
AAGAA 85 1.3917071 5.254889 75-79
TTGTT 110 1.3908576 5.850453 4
GGAGA 115 1.3812783 5.557543 3
ATGAC 110 1.3750039 6.252721 95-96
TGTTC 125 1.3730987 10.165325 5
GGGCA 140 1.3694727 9.052216 4
ATGAT 95 1.3668885 6.6574664 6
CCACT 140 1.3551775 5.3746343 30-34
TGGCT 135 1.3549163 13.931552 3
GATGG 120 1.3511539 10.419648 9
TCGTA 115 1.3475639 5.421894 40-44
TGTCA 115 1.3475639 5.421894 5
GCTGA 125 1.3382844 9.907587 6
CAGAA 100 1.3334324 5.6025352 90-94
CCAAA 105 1.3312978 5.8665853 8
GGGCT 145 1.3296387 12.728768 1
TAGGA 100 1.3146083 12.165386 4
GACAG 115 1.313397 5.2844243 1
GGTCC 150 1.3078917 8.068819 6
CCATC 135 1.3067783 8.957724 9
AAATG 85 1.3046323 7.101804 6
TTCAA 95 1.2997144 6.330293 9
CGTAT 110 1.2889742 8.675031 45-49
TGACT 110 1.2889742 5.421894 3
TATGC 110 1.2889739 8.67503 45-49
GCCCT 155 1.2850707 7.672287 3
TGGGC 140 1.283789 8.485846 7
ACTTT 100 1.2825212 5.9342256 1
ATGTT 95 1.2813665 6.2409286 1
ATTTG 95 1.2813663 12.481856 9
TGGTT 110 1.2707777 5.345353 5
TGGTG 120 1.2666163 9.767722 7
GTTTT 100 1.2644161 5.8504534 6
GCCTG 145 1.2642952 12.103229 1
TTGCT 115 1.2632507 6.0991945 50-54
CCACC 150 1.2614243 7.7821474 5
GGACA 110 1.2562928 15.853274 6
GAAGC 110 1.2562928 10.568849 9
TGACA 100 1.2500036 5.7837667 9
GACAT 100 1.2500035 11.567533 7
TGGAA 95 1.248878 6.082693 5
ACAGC 115 1.2488517 10.049455 5
AATCC 105 1.2480024 5.499531 7
TGCCT 130 1.2406145 8.831266 3
AGGTG 110 1.2385577 5.209824 4
GTGGC 135 1.2379395 12.728768 1
CATGT 105 1.2303842 5.4218936 1
TAGAT 85 1.2230055 6.0453725 90-94
CCCTC 155 1.2219174 7.295242 4
GCCGT 140 1.2206988 8.068819 3
AGTTT 90 1.2139261 6.2409286 7
TTTAG 90 1.213926 6.240928 8
TTGGG 115 1.2138406 9.767722 2
ACCTC 125 1.20998 8.957724 1
AGCAA 90 1.2000892 6.169792 9
CAAAG 90 1.2000891 6.169791 5
AAAGC 90 1.2000891 6.169791 6
ACAGG 105 1.1991886 10.568849 8
AGGCA 105 1.1991886 5.712891 95-96
ATCAG 95 1.1875033 5.7837663 6
ATGAG 90 1.1831475 6.082693 25-29
CAGTT 100 1.1717947 5.1698627 85-89
ATGCT 100 1.1717947 5.421894 8
TCAAT 85 1.1629024 6.3302937 10-14
TGTGT 100 1.1552525 10.690706 3
GCCCA 130 1.1497369 12.276536 1
TGATT 85 1.1464858 12.481857 5
TGCTC 120 1.1451827 8.831267 4
TGTCC 120 1.1451827 13.2469015 2
TCCCC 145 1.143084 7.295242 2
AAGGC 100 1.1420842 5.493164 65-69
CAACA 90 1.1411123 5.8665853 8
CACAA 90 1.1411123 11.7331705 9
ACATC 95 1.129145 5.4995303 8
AAGCT 90 1.1250031 6.2527194 95-96
GAAAG 80 1.1218792 12.977338 7
AAGGA 80 1.1218792 6.488669 3
GCACT 110 1.1198142 9.4206915 5
CCTGA 110 1.119814 9.420691 9
ACCTT 100 1.1142083 5.1554418 7
GTCAT 95 1.113205 5.421894 1
TGATC 95 1.113205 10.843788 5
TCATG 95 1.113205 5.421894 3
TGGAT 90 1.1091216 5.702118 9
GTGGG 115 1.1090435 8.924425 1
CTGTG 110 1.1040058 9.2877 4
GCTTT 100 1.0984789 5.4947696 95-96
TGTCT 100 1.0984789 10.165323 5
TTGGT 95 1.0974898 5.345353 4
CTGTC 115 1.0974668 17.662535 4
CAGAC 100 1.0859579 5.0247273 5
GGAAC 95 1.0849801 5.2844243 6
CCTCG 130 1.0778012 7.672287 6
GCGGC 135 1.075477 7.372196 1
ATAAA 60 1.0752101 8.291662 7
GGGAT 95 1.0696635 10.419649 3
CATCC 110 1.0647823 8.957723 3
ACAGT 85 1.062503 5.7837663 4
ACTGA 85 1.062503 11.567533 7
GTTGG 100 1.0555136 9.767722 1
TGTGG 100 1.0555136 9.767722 5
GGAAA 75 1.0517617 19.466007 6
GTGAA 80 1.0516868 6.082693 1
GAAGT 80 1.0516866 6.082693 5
GTCTC 110 1.0497508 8.831267 1
CGGCT 120 1.046313 8.068818 1
TTTAT 70 1.0333818 5.4645233 10-14
GACAC 95 1.0316601 10.049455 7
GGCAA 90 1.0278759 10.56885 3
TCATA 75 1.0260904 6.330293 5
ATTCA 75 1.0260903 6.3302927 7
TAACA 70 1.0216029 6.7527957 8
GGTCA 95 1.0170963 9.907589 3
ATGGC 95 1.0170962 9.907587 1
TCAGG 95 1.0170962 9.907587 8
GGTGA 90 1.0133655 15.629474 3
TGTTT 80 1.0115329 5.8504534 5
TGAAT 70 1.007181 6.6574664 5
ATTGA 70 1.0071809 6.6574664 7
AAGTT 70 1.0071809 6.6574664 6
TTGCC 105 1.0020349 8.831267 2
CTTGC 105 1.0020349 8.831267 6
GCAAA 75 1.0000744 6.169792 4
CATAG 80 1.0000029 6.2527204 95-96
GACTT 85 0.99602544 5.421894 1
CTGAT 85 0.99602544 5.421894 4
CTTGT 90 0.988631 10.165323 3
AATGG 75 0.98595625 6.082693 8
AAGGT 75 0.9859562 6.0826926 4
GATGT 80 0.98588586 5.7021174 7
GGATT 80 0.98588586 11.404235 5
GGCGG 115 0.96349704 7.753219 1
AGAGG 80 0.9608892 5.557543 8
GAGGT 85 0.95706743 5.2098246 3
ATGGG 85 0.9570673 5.209824 1
CCGTC 115 0.95343953 7.672287 4
TAGCA 75 0.9375027 5.7837667 1
ACATG 75 0.9375026 5.7837663 2
TTGCA 80 0.93743575 5.421894 4
GTTCA 80 0.93743575 5.421894 6
ATGTC 80 0.93743575 5.421894 5
TTCAG 80 0.93743575 5.421894 8
TTGAC 80 0.9374356 5.4218936 2
GTTCT 85 0.93370706 5.0826616 1
TTGTC 85 0.93370706 5.0826616 9
TTTGC 85 0.93370706 5.0826616 3
ATGGT 75 0.924268 5.7021174 4
ATGAA 60 0.920917 7.1018047 9
AGATG 70 0.92022586 6.082693 5
GCTCA 90 0.91621155 5.092265 95-96
AGTGC 85 0.9100334 9.907587 2
AGGGT 80 0.90076935 10.419649 1
GTAGG 80 0.90076923 10.419648 6
AGTGG 80 0.90076923 5.209824 2
TAAAA 50 0.89600843 8.291662 8
CACAT 75 0.89143026 5.499531 6
CCATT 80 0.89136666 10.3108835 9
ATACT 65 0.8892783 6.330293 9
ACATT 65 0.88927823 6.3302927 7
GCGGG 105 0.87971467 7.753219 2
ACACC 85 0.8777014 9.555587 9
CATAA 60 0.8756596 6.7527947 6
ACCCT 90 0.8711856 13.436585 1
GAACA 65 0.8667311 6.169792 7
ACTGC 85 0.8653109 5.092265 95-96
GGTAT 70 0.86265016 17.106354 6
AGTTG 70 0.86265016 5.702118 7
GAGAC 75 0.85656327 5.2844243 1
GTGTC 85 0.8530954 13.93155 1
GTTGC 85 0.8530954 9.2877 1
ATAGA 55 0.84417385 7.1018047 8
GAAAT 55 0.84417385 7.1018047 5
CATTC 75 0.83565605 5.155441 6
TCACA 70 0.83200157 5.499531 3
TGCGG 90 0.8252928 8.485845 3
GCATT 70 0.8202563 5.421894 4
GAACC 75 0.8144686 5.0247283 6
CTCGA 80 0.81441027 9.420691 6
GAATC 65 0.8125023 5.7837667 6
TACAG 65 0.81250226 11.567533 7
TGGTA 65 0.80103225 11.404236 5
AAGAC 60 0.80005944 6.169791 8
CAAGG 70 0.7994591 5.2844243 2
ATGTA 55 0.7913565 6.6574664 4
AATGT 55 0.7913565 6.6574664 3
CGGCA 85 0.7906042 8.607354 2
GAGAG 65 0.7807225 5.557543 8
ACCAT 65 0.7725729 5.499531 8
TTCTA 60 0.7695128 5.934226 9
TAGAA 50 0.7674308 7.1018047 9
GCATC 75 0.7635097 9.4206915 1
GTTCC 80 0.76345515 8.831267 6
AGCTT 65 0.76166654 5.421894 1
TTAGC 65 0.76166654 5.421894 9
CTGTA 65 0.76166654 5.421894 2
ACTTG 65 0.7616664 5.4218936 2
GTGCT 75 0.7527313 9.287701 3
ATCAT 55 0.7524662 6.3302927 3
GTTTG 65 0.7509141 5.345353 9
GTGTT 65 0.7509141 10.690706 1
GTCAA 60 0.75000215 11.5675335 6
AATGC 60 0.75000215 6.252721 95-96
CAAGT 60 0.7500021 5.7837663 9
GCAAT 60 0.7500021 5.7837663 4
GCAAG 65 0.74235487 5.2844243 1
AGTGT 60 0.7394144 5.7021174 1
TTAGG 60 0.7394144 5.702118 7
AGCGG 75 0.73364604 9.052214 1
ATCCT 65 0.72423524 5.155441 4
ACTCT 65 0.72423524 5.155441 9
AGTGA 55 0.7230346 6.082693 6
AATAA 40 0.71680677 8.291662 6
AACCT 60 0.71314424 5.4995303 1
ATTCT 55 0.70538664 5.9342256 7
AGTCT 60 0.7030768 5.421894 3
GTGCA 65 0.69590795 9.907589 6
AAAGT 45 0.69068766 7.101804 8
AACTG 55 0.6875019 5.7837663 1
CGAAG 60 0.68525064 5.2844243 4
GATTG 55 0.67779654 5.702118 6
GTGAT 55 0.67779654 11.404236 4
TGTTA 50 0.67440337 12.481857 5
TTGTA 50 0.6744033 6.240928 9
TATTG 50 0.6744033 6.240928 7
CTCTA 60 0.6685249 5.1554413 7
TACCT 60 0.66852486 10.310882 8
ATGGA 50 0.65730417 6.082693 8
ATACA 45 0.6567447 6.7527957 6
ATCAA 45 0.65674466 6.7527947 9
TGTAA 45 0.6474735 6.6574664 7
GCGGT 70 0.6418945 8.485846 4
GGCCG 80 0.63731974 7.372196 2
GGTTT 55 0.63538885 10.690706 9
TTGTG 55 0.63538885 5.345353 1
TATAT 40 0.62991583 7.2865515 8
CCTGT 65 0.62030727 8.831266 3
GTGAG 55 0.6192789 5.2098246 1
TAGGG 55 0.61927885 5.209824 8
GAGTT 50 0.6161787 5.7021174 6
ATGTG 50 0.6161787 5.702118 2
GAATA 40 0.61394465 7.1018047 6
CTGCG 70 0.6103493 8.068818 2
CGGTG 65 0.59604484 8.485845 2
TAAGG 45 0.5915738 6.082693 9
AAGTG 45 0.5915737 6.0826926 1
TATTT 40 0.5905039 6.8306537 8
GGCAT 55 0.5888452 14.861383 3
GTATC 50 0.5858973 5.421894 4
ATAAC 40 0.5837731 13.505591 7
TTACT 45 0.57713455 5.934226 9
GTATA 40 0.575532 13.314933 7
GAGTG 50 0.5629808 5.209824 1
GTACA 45 0.5625016 5.7837667 6
ATAGC 45 0.5625016 5.7837667 9
TCTAC 50 0.5571041 5.1554413 8
GCGAG 55 0.53800714 9.052216 1
ACGGG 55 0.5380071 9.052214 1
GATAA 35 0.5372016 7.1018047 6
AATAG 35 0.5372016 7.101805 7
CAACT 45 0.53485817 5.4995303 6
CATAC 45 0.53485817 5.4995303 5
GATTC 45 0.52730757 5.421894 6
AGGTA 40 0.5258433 12.165386 5
CGGTC 60 0.52315664 8.068819 5
ACGAG 45 0.51393795 5.2844243 7
TATTC 40 0.5130085 5.9342256 7
CTAAA 35 0.51080143 6.7527957 9
TACAA 35 0.51080143 5.402236 35-39
CCTTA 45 0.5013937 5.1554413 6
CAGTA 40 0.50000143 5.7837667 4
GTGTA 40 0.49294293 5.702118 4
TAACT 35 0.47884214 6.330293 8
CTTAA 35 0.47884214 6.330293 7
CTATA 35 0.47884214 6.330293 4
TTAAC 35 0.47884214 6.330293 8
TATCA 35 0.4788421 6.3302927 5
TCAAC 40 0.47542948 5.499531 7
ACTCA 40 0.47542942 5.49953 8
TTAGT 35 0.47208238 10.120425 95-96
TGTAT 35 0.47208238 6.2409286 3
ATTGT 35 0.47208235 6.240928 8
GTTAC 40 0.46871787 5.421894 6
TGTAC 40 0.46871787 10.843788 7
AGAGT 35 0.46011293 6.082693 5
AGTAG 35 0.46011293 6.082693 5
CTCCG 55 0.45599285 7.672287 6
GGTAG 40 0.45038468 5.2098246 2
TTTAC 35 0.44888243 5.9342256 8
CTACT 40 0.44568333 5.1554418 4
AACTA 30 0.4378298 6.7527947 9
TATAG 30 0.43164897 6.6574664 5
ATATA 25 0.4199739 7.7728767 9
CTCAA 35 0.41600078 5.499531 9
TATAC 30 0.4104361 6.3302927 5
ACTAT 30 0.4104361 6.3302927 6
TACTA 30 0.4104361 6.3302927 5
TCGAT 35 0.41012815 10.843788 7
ACGTT 35 0.41012815 5.421894 4
CGAAA 30 0.40002972 6.169792 9
GTAAG 30 0.3943825 6.082693 8
ATAGG 30 0.3943825 6.082693 3
TCCTA 35 0.38997287 5.1554413 5
TTACC 35 0.38997287 5.1554413 7
ACCGA 35 0.3800853 5.0247273 7
GCATA 30 0.37500107 5.7837667 1
TCGAA 30 0.37500107 5.7837667 4
GCTAA 30 0.37500107 5.7837667 8
TAGGT 30 0.3697072 5.7021174 7
GTTAG 30 0.3697072 5.702118 6
CAATA 25 0.36485815 6.7527947 5
ATACC 30 0.35657212 5.499531 6
GACGA 30 0.3426253 5.284424 6
AAGCG 30 0.3426253 10.568848 7
GTTTA 25 0.33720168 6.2409286 7
GTATT 25 0.33720168 12.481857 6
AGATA 20 0.30697232 7.1018047 5
CGTCA 30 0.30540386 9.420691 5
CCTAA 25 0.29714343 5.499531 7
TACCA 25 0.2971434 5.49953 9
TGCTA 25 0.29294866 5.421894 7
TACGT 25 0.29294863 5.4218936 9
AGACG 25 0.2855211 5.284425 9
CCTAT 25 0.2785521 5.1554418 3
TAAGC 20 0.25000072 5.7837667 9
CTAAG 20 0.25000072 5.7837667 8
CGATT 20 0.23435894 5.421894 9
GGGTA 20 0.22519234 5.2098246 2
ACGCA 20 0.21719159 5.0247273 5
GCGAA 15 0.17131266 5.284425 3
CGAAC 15 0.16289368 5.0247273 5
Produced by FastQC (version 0.10.1)